Regulatory

Part:BBa_K2406020:Design

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-09-27)


T7-LacO Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed this part by using PCR primers with overhangs to add the Lac Operator. Original T7 Promoter was BBa_I712074.


Source

This part was derived from T7 promoter, which is from T7 bacteriophage and LacO, which is derived from bacteria containing the Lac operon.

References